Tạp chí Khoa học, T. 98, S. 10 (2014)

Cỡ chữ:  Nhỏ  Vừa  Lớn

Inhibition abilities of Bacillus spp. to Vibrio parahaemolyticus on early mortality syndrome in Thua Thien Hue

Hồ Thị Tùng, Trần Quang Khánh Vân, Nguyễn Văn Khanh

Tóm tắt


Datum were collected on 216 samples, with 4 repetitions each 54 which collected in different shrimp culture ponds in Quang Dien and Phu Vang, Thua Thien Hue province and isolated of Bacillus strains: B. subtilis A , B and B. subtilis B. Bsubtilis BS. Under culture conditions for microbial strains Bacillus (370C incubation, pH 5-8, concentration of NaCl 1-4 %), different strains of Bacillus has tested with Vibrio. The experiments were tested with 20 strains of Vibrio, which were isolated from the EMS on shrimp. There are 4 strains of Vibrio (cholera, VK1; alginolitycus,VD2; mimicus, X1; parahaemolyticus, X2),  which were selected for pairs for inhibition of Bacillus. First results conducted the ability of Bacillus resistance to pathogenic Vibrio as (1) best concentration of Bacillus for inhibition of 107 CFU/ml with Vibrio is 105 CFU/ml and obtained 24.77 ± 0.71 mm of anti-dimension; (2) the optimal time and a highest inhibition after 20 h mixture of Bacillus and Vibrio. Simultaneously, with 12 pairs tested in 3 treatments according to Latin square design and the results were showed two pairs (A - X2) and (A - X1) with maximum anti-dimension of the inhibition to Vibrio, as 35.77 ± 0.83 and 29.83 ± 0.45 mm, respectively. The results and innovation can be announced to farmers and shrimp culture ponds to apply for the prevention of EMS in the region and country.



 

Tạp chí Khoa học - Đại học Huế

04 Lê Lợi, TP. Huế

Hue University Journal of Science